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VM244: Diagnostic Methods for the Comprehensive Health Assessment of the Long-Spined Sea Urchin, Diadema antillarum

Figure 17. Collecting coelomic fluid from juvenile D. antillarum. A 1-inch, 21 gauge needle with an attached 3 mL syringe can be used to collect samples. The needle is inserted at an angle of 70°, directed away from the midline, and kept shallow to avoid contamination with gastrointestinal contents. Note that the striped spines visible on this animal suggest juvenile status. Also note that the sample is being collected without trimming the spines in a procedure which would be considered nonlethal. The animal is inverted and restrained with the tongs, allowing coelomic fluid collection without causing injury. This animal was not sedated for this procedure. MS-222 is an effective sedative to facilitate handling.

Credit: R. Francis-Floyd, UF/IFAS