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VM244: Diagnostic Methods for the Comprehensive Health Assessment of the Long-Spined Sea Urchin, Diadema antillarum

Figure G2-1. Test and appendages. (a) External view of the test ambulacrum of oral area. (b) External view of the test ambulacrum of aboral area. (c) External view of the test interambulacrum of aboral area. (d) External view of the test interambulacrum of aboral area. (e) Internal view of the test ambulacrum of oral area showing ampulla. (f) Internal view of the test ambulacrum of aboral area showing ampulla. (g) Internal view of the test interambulacrum of oral area after the intestine removed. (h) Internal view of the test interambulacrum of aboral area after the gonad removed. (i) Internal view of the test interambulacrum of aboral area before the gonad (histologically confirmed as ovary) removed. Some spines were still not completely decalcified in this specimen. (j) Internal view of the test interambulacrum of oral side after the intestine and gonad removed. (k) Same view of (j) but the test can be easily longitudinally sectioned with scissors (indicated with dashed white line) because the spines were completely decalcified for this specimen. (l) External view of the aboral side of the test ambulacrum plate. (m) External view of the aboral side of the test interambulacrum plate. (n) External view of the aboral area of test plates of juvenile sample exhibiting with blue-violet iridophores radiate around the ambulacral plate. (o) Internal view of (n). (p) External view of oral side of the test interambulacrum plate with spines having peculiar water-soluble greenish pigments. (q) Spines of (p) removed. (r) Spines and pedicellariae from aboral side of the test of ambulacrum plate. (s) Melanin pigments are commonly found at the center core of the spine, especially at the shaft portion (e.g., see histology figure, Fig. H1o).

Credit: Yasunari Kiryu, FWC/FWRI