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VM244: Diagnostic Methods for the Comprehensive Health Assessment of the Long-Spined Sea Urchin, Diadema antillarum

Figure G9-1. Gross recognizable abnormalities (some pathologies) shown. (a) Gill that had oocytes adhered at the surface of mature female specimen. (b) Enlarged view of (a). (c) Test with oocytes adhered at the surface of mature female specimen. Inset of (c) showing high magnification of oocytes (white thick arrow). (d) Test external view. (e) Test internal side view of (d). (f) Tissue wet mount taken from the oocytes adhered to the test surface of (c). Inset of (f) showing high magnification of the tissue wet mount of oocytes taken from the test surface of (d). Note that these oocytes possibly originated from fully mature female Diadema (see histology, disease, and abnormalities section). (g) Normal view of peristomial membrane of juvenile. (h–i) Artifact needle puncture mark (hollow white arrow) of juvenile specimens. (j) Brown pigmentation in the ampullae of ambulacrum test plate of aboral side. (k) Brown pigmentation in the ampullae of ambulacrum test plate of oral side. (l) Longitudinally sectioned view of the ambulacrum test plate showing the brown pigmentation in the ampullae. (m) Spine tubercle shown where the spine itself was broken or pulled out. (n) Same specimen as (m), but the area had two spine tubercles, and healing occurred indicated by shiny appearance. (o) Brown pigmentation in the hemal vessel of small intestine. (p) Brown pigmentation in the hemal vessel of both small and large intestine border. Ampu = ampulla; Arrow head = spine tubercle; Hollow white arrow = needle puncture mark; LI = large intestine; SI = small intestine; Sp = spine; White thick arrow = egg mass; White thin arrow = brown pigmentation.

Credit: Yasunari Kiryu, FWC/FWRI