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ENY-167: How to Quantify Nosema Spores Infection Rate in a Honey Bee Colony

Figure 4. Diagram of the viewing grid of a hemocytometer. Note that there are 25 blocks that (bordered on all sides by 3 lines) each contains 16 smaller squares. The total number of the Nosema spores present in five of these blocks should counted. We have labeled 9 of the blocks in this diagram and suggest that you count spores in five of these blocks as we describe in the text above (step 11 of the Nosema Quantification section).

Credit: UF/IFAS Honey Bee Lab