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VM244: Diagnostic Methods for the Comprehensive Health Assessment of the Long-Spined Sea Urchin, Diadema antillarum

Figure H8-1. Histopathological section of the gill and test. (a) Tangential section of gill. (b) Higher magnification of the hemal vessel of the gill showing needle-like structures (spicule) associated with coelomocyte aggregates. (c) High-power magnification of (a) showing basophilic cellular aggregates (sphere) in hemal vessel. Inset shows Giemsa stain. (d) Bacterial colonies found at the oral side of the test, internally. (e) Bacterial colonies with high-power magnification of (d). (f) Gram-negative, short rod bacteria demonstrated with Gram stain. Ampu = ampullae; Arrows, thin = needle-like objects; Arrow, thick hollow = bacterial colonies; Arrow head, hollow = basophilic cellular aggregates; HV = hemal vessel; NC = nerve cord; Sp = spine.

Credit: Jan Landsberg and Yasunari Kiryu, FWC/FWRI