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African Malaria Mosquito Anopheles gambiae Giles (Insecta: Diptera: Culicidae)1

Sabrina A. White and Phillip E. Kaufman2

Introduction

Anopheles gambiae Giles is the most efficient vector of human malaria in the Afrotropical Region (CDC 2010). Thus, it is commonly called the African malaria mosquito. The Anopheles gambiae complex of sibling species (White 1974; Fanello et al. 2002; Coetzee et al. 2013) comprises eight reproductively isolated species that are almost indistinguishable morphologically: Anopheles amharicus Hunt et al. 2013, Anopheles arabiensis Patton 1905, Anopheles bwambae White 1985, Anopheles gambiae Giles 1902, Anopheles coluzzii Coetzee & Wikerson 2013, Anopheles melas Theobald 1903, and Anopheles merus Dönitz 1902. Collectively they are sometimes called Anopheles gambiae sensu lato, meaning ‘in the wider sense.’ None of these species occur in North America.

Figure 1. 

Female Anopheles gambiae Giles taking a blood meal.


Credit:

Jim Gathany, CDC.


[Click thumbnail to enlarge.]

Synonymy

Anopheles gambiae Giles, 1902 sensu stricto (White 1975; Mattingly 1977)

Anopheles gambiae species A (Davidson et al. 1967; White 1974)

Anopheles costalis Loew 1866 nomen dubium

(From ITIS 2013)

Distribution

Members of the Anopheles gambiae complex are found throughout tropical Africa (WHO 1989), south of the Sahara desert, with Anopheles arabiensis extending across southern Arabia. Anopheles gambiae s.s. is distributed throughout sub-Saharan Africa, including Madagascar (WHO 1989).

Figure 2. 

The approximate distribution of Anopheles gambiae s.s. Giles in tropical Africa. Diagram by Sabrina White, University of Florida, based on maps and data from Sinka et al. 2010.


[Click thumbnail to enlarge.]

Description

Adults

Adult female Anopheles can be differentiated from other mosquito genera because the palps (appendages found near the mouth) are as long as their proboscis (feeding tube) (Foster and Walker 2009). Adult Anopheles also have a distinguishable resting position where their abdomen is raised into the air (Foster and Walker 2009). Anopheles gambiae have a variable body color, but it typically ranges from light brown to grey with pale spots of yellow, white or cream scales, and dark areas on their wings (Gillies and de Meillon 1968). In comparison to other species, adults are considered small- to medium-sized mosquitoes with average wing length varying from 2.8 to 4.4 mm (Gillies and de Meillon 1968).

Figure 3. 

Adult female African malaria mosquito, Anopheles gambiae Giles.


Credit:

Lyle Buss, University of Florida.


[Click thumbnail to enlarge.]

Figure 4. 

Female Anopheles gambiae Giles taking a blood meal.


Credit:

James Gathany, CDC.


[Click thumbnail to enlarge.]

Eggs

Eggs are between 0.47 and 0.48 mm long, convex below and concave above, and the surface is covered with a polygonal pattern (Gillies and de Meillon 1968). Similar to other Anopheles species, Anopheles gambiae lay their eggs singly and directly on the water, with each egg having floats on either side (Foster and Walker 2009). Anopheles eggs are not drought resistant (CDC 2010).

Figure 5. 

Unhatched (left) and hatched (right) eggs of Anopheles stephensi Liston, showing the characteristic singly laid Anopheles eggs with floats on both sides, and the split where the larva emerged.


Credit:

Mark Benedict, CDC.


[Click thumbnail to enlarge.]

Larvae

All Anopheles larvae lack the respiratory siphons used as breathing tubes in most other mosquito genera, and therefore the larvae lie parallel to the water surface in order to breathe (Foster and Walker 2009). Anopheles mosquitoes develop though four larval sizes or instars before pupating (Foster and Walker 2009). Larvae are very small in the first instar and increase in size until reaching 5 to 6 mm by the completion of the fourth instar (Gillies and de Meillon 1968). They feed on organic matter and algae (Garros et al. 2008).

Figure 6. 

Larva of the African malaria mosquito, Anopheles gambiae Giles.


Credit:

Ray Wilson, Bird and Wildlife Photography


[Click thumbnail to enlarge.]

Pupae

Pupae of all mosquitoes are comma-shaped when viewed from the side (CDC 2010). Unlike the pupae of many other insects, mosquito pupae are very mobile; they use the paddle at the end of their abdomen to quickly move through their aquatic habitat (Foster and Walker 2009). The pupal stage does not feed (Foster and Walker 2009).

Figure 7. 

Pupa of the African malaria mosquito, Anopheles gambiae Giles.


Credit:

Ray Wilson, Bird and Wildlife Photography


[Click thumbnail to enlarge.]

Life Cycle and Biology

As with all mosquitoes, Anopheles gambiae has four life stages: egg, larva, pupa, and adult. Both male and female adult mosquitoes feed on nectar from plants, but only the female bloodfeeds on vertebrates, where she obtains nutrients for her eggs (Foster and Walker 2009). Although adults can survive for up to one month in captivity, they usually survive around one to two weeks in the wild (CDC 2010).

Anopheles gambiae adults are active at night, with peak hours of activity from after midnight to 4:00 am, with activity continuing until just before dawn (Gillies and de Meillon 1968). The females prefer to bloodfeed indoors, but outdoor feeding also occurs (Tuno et al. 2010). Anopheles gambiae are anthropophilic, meaning they prefer to bloodfeed on humans as opposed to other animals (White 1974). This makes them very efficient vectors of the human malaria parasites and contributes to their status as one of the most important malaria vectors in the world (CDC 2010).

Adult females lay their eggs on the surface of water in a variety of aquatic habitats, but prefer shallow sunlit pools of standing water (Gillies and de Meillon 1968). Larvae hatch from eggs and develop within the aquatic habitat. Studies have shown that Anopheles gambiae larvae can develop in permanent man-made structures such as concrete tanks and drainage canals (Mala et al. 2011) and natural pools such as swamps, hoof prints (Kweka et al. 2012), and marshes (Mala et al. 2011).

As for all mosquitoes, the larvae of Anopheles gambiae pupate after the fourth instar once they acquire an appropriate amount of nourishment (Foster and Walker 2009). The pupal stage is a non-feeding, mobile stage, during which the mosquito’s adult body is formed. Anopheles gambiae can develop from egg to adult in 10–11 days, but development is temperature dependent and can take as long as three weeks under colder conditions (Gillies and de Meillon 1968).

Seasonal abundance of Anopheles gambiae varies depending on location, but generally the population decreases during the dry season and peaks during the wet season (Gillies and de Meillon 1968, Yaro et al. 2012). Populations begin to increase as the rainy season commences, peak in mid-season, and decline as water levels stabilize and aquatic predators establish themselves (Gillies and de Meillon 1968).

Figure 8. 

An example of the sunlit pools that are ideal larval habitats for Anopheles gambiae Giles


Credit:

CDC


[Click thumbnail to enlarge.]

Medical Significance

In Africa in 2010, there were an estimated 174 million cases of malaria and 596,000 deaths (WHO 2012). Approximately 90% of all malaria deaths occur in Africa (WHO 2012). Anopheles gambiae are considered to be one of the world’s most important human malaria vectors because of their susceptibility to the Plasmodium parasite, their preference for humans as a host, and their indoor-feeding behavior (CDC 2010). Due to their short development time and their preference for developmental habitats near human dwellings, Anopheles gambiae are considered effective vectors of human malaria, as well as lymphatic filariasis (elephantiasis).

Malaria

It is important to note that the mosquitoes themselves do not cause the malaria disease. Malaria is caused by a protozoan parasite in the Plasmodium genus that the mosquitoes transmit through blood feeding (Foster and Walker 2009). Different species of Plasmodium infect different vertebrate groups, including humans, other mammals, birds, and reptiles. Not only is each Plasmodium specific for the vertebrate, many are transmitted to the non-human vertebrate by different mosquito species; for example, mosquitoes in the Culex genera are important vectors for transmission of Plasmodium among birds (Foster and Walker 2009). Human malaria is caused by four human-specific species of Plasmodium: Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale, and Plasmodium vivax (WHO 2013), plus one zoonotic species, Plasmodium knowlesi, known from monkeys in southeastern Asia. The parasites are transmitted in the saliva of female Anopheles mosquitoes (Foster and Walker 2009). When a female Anopheles mosquito takes a blood meal from an infected human, she ingests the Plasmodium parasite and, after a certain developmental time within the mosquito, she can transmit the parasite through subsequent blood feedings (Foster and Walker 2009).

The human malaria disease is characterized by sudden fever and chills at regular intervals. Other symptoms can include fatigue, headache, and sweating after each fever (Foster and Walker 2009). Malaria is no longer transmitted regularly in the United States; however, historically it was an important disease. More information can be found on historical malaria transmission in the U.S. in the Anopheles quadrimaculatus Featured Creatures article.

Figure 9. 

Three pink crescent shaped Plasmodium falciparum gametocytes, which are the cells that divide to form reproductive cells.


Credit:

Dr. Mae Melvin, CDC.


[Click thumbnail to enlarge.]

Figure 10. 

Swelling of the abdomen, eyes, feet and hands are a common symptom of kidney damage associated with infection by one type of malaria, Plasmodium falciparum.


Credit:

Dr. Myron Schultz, CDC.


[Click thumbnail to enlarge.]

Management

Various management techniques are used to control mosquito populations and limit malaria transmission in an area. Effective and currently used management practices include education of the community about malaria and the role of mosquitoes in transmission, house and environment modifications to prevent mosquito entry and to reduce larval development site availability, and the use of bed nets, spatial repellents, and indoor residual spraying (IRS) of insecticides (Takken and Knols 2009). Proposed management practices include the introduction of biological controls such as predators, sterile insect technique (SIT), and the release of genetically-modified mosquitoes (Takken and Knols 2009).

Greatly assisted by multiple organizations such as The President’s Malaria Initiative and The Bill and Melinda Gates Foundation, the distribution of insecticide-treated bed nets in Africa has profoundly decreased the incidence of malaria (CDC 2012). About 145 million treated bed nets were delivered to sub-Saharan Africa in 2010 alone (CDC 2012).

Heavy reliance on insecticides in an area can lead to the development of insecticide resistant mosquito populations (Brogdon and McAllister 1998, Mawejje et al. 2013). Studies in eastern Uganda have found that Anopheles gambiae are extremely resistant to pyrethroid insecticides. Such high resistance levels could decrease the efficacy of pyrethroid treated bed nets (Mawejje et al. 2013). For this reason, studies monitoring resistance to insecticides in mosquito populations should be conducted regularly.

Figure 11. 

Digging drainage canals to remove standing water is one method to reduce mosquito larval developmental opportunities.


Credit:

CDC


[Click thumbnail to enlarge.]

Modifications of the home and environment also can lead to dramatic changes in mosquito abundance (Takken and Knols 2009). Screens placed over doors and windows can prevent mosquito entrance, reducing malaria cases in an area (Takken and Knols 2009). Making small environmental changes to decrease the number of breeding sites also can lead to substantial decreases in mosquito populations (Takken and Knols 2009). The periodic draining of rice fields and fish ponds was a very effective method used in Java, Indonesia (Takken and Knols 2009).

Selected References

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Carpenter SJ, Middlekauff WW, Chamberlain RW. 1946. The Mosquitoes of the Southern United States East of Oklahoma and Texas. The American Midland Naturalist Monograph Series No. 3. University Press. Norte Dame. 292 pp.

CDC. (2004). Anopheles mosquitoes. Malaria. http://www.cdc.gov/Malaria/biology/mosquito/index.htm (11 September 2007).

CDC. (2005). CDC Entomology Branch—Overview of Current Activities. West Nile Virus. (11 September 2007).

Connelly R. (2007). Mosquito Information website. http://mosquito.ifas.ufl.edu/ (12 September 2007).

Efird PK, Inman AD, Weathersbee AA, Meisch MV. 1992. Efficacy of various ground-applied pyrethroids against adult Anopheles quadrimaculatus in the rice growing region of Arkansas. Journal of the American Mosquito Control Association 8: 77–79.

Fasulo TR. (2002). Mosquitoes 1 and Mosquitoes 2. Bug Tutorials. University of Florida/IFAS. CD-ROM. SW 160.

Jensen T, Kaiser PE, Barnard DR. 1994. Adaptation to intermittently flooded swamps by Anopheles quadrimaculatus species C1 (Diptera: Culicidae). Environmental Entomology 23: 1150–1154.

Jensen T, Cockburn AF, Kaiser PE, Barnard DR. 1996. Human blood-feeding rates among sympatric sibling species of Anopheles quadrimaculatus mosquitoes in northern Florida. American Journal of Tropical Medicine and Hygiene 54: 523–525.

Kramer VL, Gargia G, Colwell AE. 1987. An evaluation of the mosquitofish, Gambusia affinis, and the inland silverside, Menidia beryllina, as mosquito control agents in California wild rice fields. Journal of the American Mosquito Control Association 3: 626–632.

Lacey LA, Inman A. 1985. Efficacy of granular formulations of Bacillus thuringiensis (H-14) for the control of Anopheles larvae in rice fields. Journal of the American Mosquito Control Association 1: 38–42.

Magnarelli LA. 1975. Life history and physiological age studies of Anopheles quadrimaculatus Say in central New York State. Proceedings of the New Jersey Mosquito Control Association 83–89.

McLaughlin RE, Vidrine MF, Willis OR. 1987. Distribution of Anopheles quadrimaculatus and An. crucians larvae within rice field habitats in Southwestern Louisiana. Journal of the American Mosquito Control Association 3: 574–578.

Moncayo AC, Edman JD, Turell MJ. 2000. Effect of eastern equine encephalomyelitis virus on the survival of Aedes albopictus, Anopheles quadrimaculatus, and Coquillettidia perturbans (Diptera: Culicidae). Journal of Medical Entomology 37: 701–706.

Nayar JK, Sauerman Jr DM. 1975. Physiological basis of host susceptibility of Florida mosquitoes to Dirofilaria immitis. Journal of Insect Physiology 21: 1965–1975.

O'Malley CM. 1992. The biology of Anopheles quadrimcaulatus Say. Proceedings of the New Jersey Mosquito Control Association 79: 136–144.

Reinert JF, Kaiser PE, Seawright JA. 1997. Analysis of the Anopheles (Anopheles) quadrimaculatus complex of sibling species (Diptera: Culicidae) using morphological, cytological, molecular, genetic, biochemical, and ecological techniques in an integrated approach. Journal of the American Mosquito Control Association 13 (Supplement) 1–102.

Robert LL, Santos-Ciminera PD, Andre RG, Schultz GW, Lawyer PG, Nigro J, Masuoka P, Wirtz RA, Neely J, Gaines D, Cannon CE, Pettit D, Garvey CW, Goodfriend D, Roberts DR. 2005. Plasmodium-infected Anopheles mosquitoes collected in Virginia and Maryland following local transmission of Plasmodium vivax malaria in Loudoun County, Virginia. Journal of the American Mosquito Control Association 21: 187–193.

Robertson LC, Prior S, Apperson CS, Irby WS. 1993. Bionomics of Anopheles quadrimaculatus and Culex erraticus (Diptera: Culicidae) in the Falls Lake Basin, North Carolina: seasonal changes in abundance and gonotrophic status, and host-feeding patterns. Journal of Medical Entomology 30: 689–698.

Rutledge RC, Baker RH, Morris CD, Nayar JK. (2005). Human malaria. EDIS. http://edis.ifas.ufl.edu/MG103 (12 September 2007).

Weathersbee III AA, Meisch MV. 1988. An economical lightweight portable resting unit for sampling adult Anopheles quadrimaculatus populations. Journal of the American Mosquito Control Association 4: 89–90.

Weidhass DE, Woodard DB, Schmidt CH, Ford HR. 1965. Biology of Anopheles quadrimaculatus under field conditions in central Florida. Annals of the Entomological Society of America 58: 145–150.

Footnotes

1.

This document is EENY601, one of a series of the Department of Entomology and Nematology, UF/IFAS Extension. Original publication date September 2014. Reviewed September 2017. Visit the EDIS website at http://edis.ifas.ufl.edu. This document is also available on the Featured Creatures website at http://entomology.ifas.ufl.edu/creatures.

2.

Sabrina A. White, undergraduate student, and Phillip E. Kaufman, professor; Department of Entomology and Nematology, UF/IFAS Extension, Gainesville, FL 32611.


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