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Nematode Management in Residential Lawns1

William T. Crow 2

A well-maintained lawn can help beautify our environment, reduce water runoff, and reduce water and air pollution. In 1992 there were 3.3 million acres of grass grown as home lawns in Florida, making home lawns the largest acreage crop in the sunshine state. For grass, just like any other crop, pest management is crucial for proper plant health. Plant-parasitic nematodes (Figure 1) are among the least understood and most difficult pests to manage on turfgrass in Florida.


Figure 1. Diagram of a generic plant-parasitic nematode.
Figure 1.  Diagram of a generic plant-parasitic nematode.
Credit: R. P. Esser, Florida Department of Agriculture and Consumer Services, Division of Plant Industry; used with permission.


Plant-Parasitic Nematodes

Nematodes are unsegmented roundworms, different from earthworms, flatworms, and other worms that are more easily observed. Nematodes living in soil are very small, and most can only be seen with the aid of a microscope (Figure 2). Many kinds of nematodes live in the soil in a home lawn. Most soil nematodes are beneficial, feeding on bacteria, fungi, or other microscopic organisms found in soil. In addition, some nematodes can be used as biological control organisms to help manage important insect pests. Unfortunately, other nematodes are harmful to plants because they feed on plant tissues and cause plant damage.


Figure 2. Size of a lance nematode (one of the largest plant-parasitic nematodes) compared to a human hair.
Figure 2.  Size of a lance nematode (one of the largest plant-parasitic nematodes) compared to a human hair.
Credit: W. T. Crow, UF/IFAS


All plant-parasitic nematodes have a stylet or "mouth-spear" that is similar in structure and function to a hypodermic needle (Figure 3 a and 3b). The nematode uses its stylet to puncture plant cells, inject digestive juices, and ingest plant fluids. All plant-parasitic nematodes that are important turfgrass pests in Florida feed on roots. Some plant-parasitic nematodes remain in the soil and feed by inserting their stylet into the root. These are called ectoparasitic nematodes (Figure 4). Others, using their stylet to puncture an entry hole in the root, feed with their body inside the root tissue. These are called endoparasitic nematodes (Figure 5). Of the common nematodes that cause damage to turfgrasses in Florida, sting, awl, stubby-root, sheath, sheathoid, spiral, and ring nematodes are strictly ectoparasites. Root-knot, lance, and lesion nematodes are endoparasites.


Figure 3. a) Retracted stylet of a lance nematode. b) Partially extended stylet of a sting nematode.
Figure 3.  a) Retracted stylet of a lance nematode. b) Partially extended stylet of a sting nematode.
Credit: J. E. Luc, UF/IFAS



Figure 4. Ectoparasitic sting nematodes feeding on a root.
Figure 4.  Ectoparasitic sting nematodes feeding on a root.
Credit: J. O. Becker, UC Riverside



Figure 5. Migratory endoparasitic lance nematodes tunneling within a root.
Figure 5.  Migratory endoparasitic lance nematodes tunneling within a root.
Credit: A. C. Hixson, UF/IFAS


Effects and Symptoms of Nematodes on Lawn Grasses

All of the plant-parasitic nematodes that damage turf in Florida feed on roots. As plant-parasitic nematodes feed, they damage plant root systems and reduce their ability to obtain water and nutrients from the soil. This makes the turf more susceptible to drought and other stresses. Nematode-damaged roots may be abnormally short and stubby (Figure 6) or appear darkened or rotten when damaged by plant-parasitic nematodes (Figure 7). Often the roots will appear "cropped off" an inch or less below the soil surface (Figure 8). Root galls or knots caused by certain species of nematodes to other crops are usually not evident on grasses but may occur in some cases (Figure 9).


Figure 6. Roots damaged by ectoparasitic nematodes may be abbreviated or
Figure 6.  Roots damaged by ectoparasitic nematodes may be abbreviated or "stubby" in appearance.
Credit: W. T. Crow, UF/IFAS



Figure 7. Healthy roots on left are white and have fine feeder roots. Roots damaged by lance nematode on right are dark, rotten in appearance, and have no feeder roots.
Figure 7.  Healthy roots on left are white and have fine feeder roots. Roots damaged by lance nematode on right are dark, rotten in appearance, and have no feeder roots.
Credit: R. A. Dunn, UF/IFAS



Figure 8. Roots of nematode-damaged turf may appear
Figure 8.  Roots of nematode-damaged turf may appear "cropped off" an inch or less below the surface.
Credit: W. T. Crow, UF/IFAS



Figure 9. Root-knot nematode galls on turf roots are often difficult to see.
Figure 9.  Root-knot nematode galls on turf roots are often difficult to see.
Credit: W. T. Crow, UF/IFAS


When nematode population densities get high enough, or when environmental stresses occur, above-ground symptoms may become evident. Symptoms include yellowing, wilting, browning, or thinning out. Grass will die under extreme nematode and environmental stress. Often, as the grass thins out spurge and other weeds may become prominent (Figure 10). Nematode damage usually appears as irregularly shaped declining areas that may enlarge slowly over time (Figure 11). Nematode symptoms on turfgrasses are often misdiagnosed because they appear similar to other factors such as localized soil conditions, fungal diseases, or insects.


Figure 10. Weeds proliferate in this zoysia turf weakened by plant-parasitic nematodes.
Figure 10.  Weeds proliferate in this zoysia turf weakened by plant-parasitic nematodes.
Credit: W. T. Crow, UF/IFAS



Figure 11. Nematode damage to this St. Augustinegrass lawn occurs in irregularly shaped patches.
Figure 11.  Nematode damage to this St. Augustinegrass lawn occurs in irregularly shaped patches.
Credit: W. T. Crow, UF/IFAS


Grass Species–Specific Associations

All types of grasses are affected by nematodes to some extent. However, certain nematodes are more problematic on some grasses than others.

Bahiagrass (Paspalum notatum) is the most tolerant turfgrass to plant-parasitic nematodes and rarely suffers damage from them. However, root-knot nematodes have recently been observed causing extensive damage to bahiagrass lawns.

St. Augustinegrass (Stenotaphrum secundatum) is the most common species of turfgrass used in Florida, and 'Floratam' is the most common cultivar of St. Augustinegrass. The most common nematodes affecting St. Augustinegrass are lance nematode, stubby-root nematode, and root-knot nematode. Floratam is relatively tolerant of sting nematode due to its thick root structure, but cultivars with finer roots are more susceptible.

Zoysiagrass (Zoysia spp.) is commonly damaged by sting, root-knot, stubby-root, and lance nematodes. No differences in nematode tolerance among zoysia cultivars have been identified.

Bermudagrass is commonly damaged by sting, root-knot, stubby-root, and lance nematodes. UF research found that among bermudagrass cultivars used in Florida, 'Celebration' and 'Princess 77' were more tolerant to sting nematode than 'Tifway' due to their vigorous rooting habit.

Centipedegrass (Eremochloa ophiuroides) is the most susceptible turfgrass to ring nematode and also is commonly damaged by sting, root-knot, stubby-root, and lance nematodes.

Seashore paspalum (Paspalum vaginatum) is fairly tolerant to most nematodes due to its vigorous root growth. However, it is very susceptible to spiral nematode. It also is occasionally damaged by sting and lance nematodes.

Nematode Diagnosis

With any plant problem, accurate diagnosis is important to address the problem and to avoid wasting time, money, effort, and pesticide applications. The only reliable way to determine if plant-parasitic nematodes are associated with grass damage is by having a nematode assay conducted by a professional nematode diagnostic lab. The University of Florida Nematode Assay Lab (NAL) is such a facility. Forms and instructions for submitting nematode samples to the Florida Nematode Assay Lab can be found on the NAL webpage at

A nematode assay requires different sampling guidelines than those required for soil analysis or disease samples. When a disease sample is submitted to most labs, a nematode analysis is not normally performed unless specifically requested. A nematode assay often requires separate payment and may even be sent to a separate address. Familiarize yourself with the procedures required by the lab where you intend to submit the sample. The accuracy of the diagnosis depends on the quality of the sample that you submit. Following the guidelines below will help ensure an accurate diagnosis:

  1. A sample must consist of multiple soil cores. Nematodes are not evenly distributed in soil, but their numbers may be high at one spot and low just a few feet way. By collecting multiple cores with a device such as a "T" type soil sample tube, an average population density can be measured. A good rule of thumb is to have 12–16 cores per lawn. Cores should be taken to a depth of 4 inches.

  2. If damage is evident, then sample near the margin of the affected area (Figure 12). Nematode populations will decline in severely damaged areas because they have nothing left to eat. Therefore, populations tend to be highest near the edges of a declining area where the grass is still alive. If damage is occurring in multiple areas of a field, take a few cores from the border of several affected areas to make the 12–16 cores. When taking samples from turf that is not showing symptoms, or if sampling before planting, sample in a "zig-zag" pattern across the area (Figure 13).

  3. Put the soil from each lawn into a separate plastic bag and seal it. Nematodes require moisture to survive, so drying the soil will kill them. This is different than submitting a sample for nutrient analysis where dry soil is preferred. Make sure that each bag is labeled with a permanent marker so that the diagnosis can be assigned to the correct area. If using a self-sealing bag, seal it with tape also because the zippers often come open in transit.

  4. Handle samples carefully. Do not expose samples to direct sunlight or heat. Nematodes are sensitive to high temperatures and UV light. Leaving samples on the dashboard or in the back of a pickup truck can kill them quickly and reduce the accuracy of the diagnosis. Keeping the nematode sample in a cooler is best. The nematodes will be sandwiched between soil particles so rough handling will destroy them. Pack the samples well to minimized shifting during shipping.

  5. Submit the sample right away. Next day delivery is best. One study found greatest nematode recovery from hand-delivered samples, the next highest from next-day delivery, and the lowest from regular postal delivery.


Figure 12. When sampling symptomatic turf, take multiple soil cores from symptomatic areas while avoiding areas of dead grass. Sample grass that is sick, not dead.
Figure 12.  When sampling symptomatic turf, take multiple soil cores from symptomatic areas while avoiding areas of dead grass. Sample grass that is sick, not dead.
Credit: W. T. Crow, UF/IFAS



Figure 13. When taking a predictive sample before symptoms occur, collect cores in a
Figure 13.  When taking a predictive sample before symptoms occur, collect cores in a "zig-zag" pattern across the field.
Credit: W. T. Crow, UF/IFAS


The NAL staff will identify any plant-parasitic nematodes found, count them, and determine if nematodes are a potential problem. Not all plant-parasitic nematodes are equal in their ability to damage grass. For example, one sting nematode can cause damage equal to hundreds of individuals of other types of plant-parasitic nematodes. The number of each type of nematode in 100 cc of soil from the submitted sample will be used to determine the risk level for the turf species indicated. The risk level will tell if the turf is at low, moderate, or high risk of damage from plant-parasitic nematodes. Be aware that different diagnostic labs may use different extraction techniques, different quantities of soil, or different thresholds. Because of this, samples submitted to separate labs may report different quantities of nematodes. In most cases the different thresholds used are adjusted to account for the differences in methodology and local conditions. However, if using a lab located in a geographically distant location, local conditions or regional variations in nematode aggressiveness may not be taken into account. Local labs will provide the most accurate assessments.

Nematode Management

Cultural Practices

Turf infested with plant-parasitic nematodes can often persist with no visible damage. Damage usually becomes evident when one of two things occur; 1) some other factor increases the susceptibility of the grass to nematode damage and/or 2) some factor causes nematode population densities to increase to damaging levels. Once the grass is planted, the best way to reduce the likelihood of nematode damage is to minimize these factors as much as possible. Therefore, some of the best practices for managing nematode damage in home lawns target avoiding other stresses on the grass. Properly irrigated and fertilized grass can often withstand higher levels of nematode infestation than grass suffering from drought or nutrient deficiencies. Following University of Florida Best Management Practices should ensure a properly cared-for lawn.

Organic amendments such as Comand® compost, mulch, municipal sludge, and manure can help the grass tolerate nematode damage by improving plant health. Remember that anything that can be done to improve root health will help plants tolerate nematode damage. In addition, some organic amendments may stimulate soil microbes that can help suppress nematodes in some situations. Over-compaction reduces oxygen availability to the root system and enhances susceptibility to nematode damage. Regular aeration encourages a healthy root system and thereby enhances tolerance to nematodes. If areas are in partial shade, trimming or thinning trees to increase light to the turf will greatly enhance the turf's ability to withstand nematode damage. Too much shade may also mean that the site is not suitable for a dense stand of turfgrass. UF research has shown that overseeding can increase nematode numbers on bermudagrass during transition by providing an alternate food source to the nematodes during the winter. Therefore, it is best to avoid overseeding when possible from a nematode management standpoint.

Pesticides and Biopesticides


The active ingredient of Indemnify nematicide is fluopyram, which is also a fungicide. Indemnify is very effective against most of the nematodes that damage turfgrasses in Florida and is labeled for all turf use sites, including golf courses, athletic fields, sod farms, and lawns. Unlike other nematicides, it has very long residual activity and can provide nematode suppression for months after application. The maximum broadcast rate for Indemnify on residential lawns is 15.5 fluid ounces per acre per year. However, areas of 10,000 ft2 or less (per acre) may be "spot-treated" up to 4 times per year at 15.5 fluid ounces per acre. Because of its fungicide activity, Indemnify should be considered in the FRAC group 7 for resistance management. While Indemnify has provided excellent control of root-knot, sting, and other nematodes in University of Florida trials, it has not been shown effective against lance nematode.

Other Products

The University of Florida provides recommendations based on research trial results for efficacy and consistency. The products mentioned above have each been evaluated extensively. However, there are a number of other products that can be used legally for nematode management in lawns. Many of these are biologically derived. Some have been evaluated and not proven effective in UF research trials. Other products either have not been evaluated or are still being evaluated. That some products are not included here does not mean a specific product is ineffective, just that we do not have enough research evidence to say that it is effective.


At present, the best management strategies for nematodes in the home lawn are reducing stress, promoting root vigor, and choosing tolerant grasses, each of which increases the turf's ability to tolerate nematode damage. Researchers at the University of Florida are continually looking at new management options and are committed to bringing you the most current management recommendations possible. Consequently, this document will be modified with each breaking development. The most current version of this document may be obtained at your county Cooperative Extension office or found online at the University of Florida's Electronic Document Information System (EDIS) website at

Additional Information

For additional information regarding nematodes, nematode management, or help interpreting nematode assay results contact:

Dr. W. T. (Billy) Crow, Professor of Nematology, Phone: (352) 273-3941, Email:, website:

For information on submitting samples to the UF/IFAS Florida Nematode Assay Lab or to check on the status of a sample you submitted contact:

UF/IFAS Florida Nematode Assay Lab, Phone: (352) 392-1994, Email:, website:


1. This document is ENY-006, one of a series of the Department of Entomology and Nematology, UF/IFAS Extension. Original publication date March 1997. Revised March 2007, April 2013, January 2014, June 2017 and July 2020. Visit the EDIS website at
2. William T. Crow, professor, Department of Entomology and Nematology; UF/IFAS Extension, Gainesville, FL 32611.

The use of trade names in this publication is solely for the purpose of providing specific information. UF/IFAS does not guarantee or warranty the products named, and references to them in this publication do not signify our approval to the exclusion of other products of suitable composition. Use pesticides safely. Read and follow directions on the manufacturer's label.

Publication #ENY-006

Release Date:November 13th, 2020

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Crow, William T


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