Sturgeon Aquaculture—Specialized Techniques: Determining the Stage of Sexual Maturity in Female Sturgeon for Artificial Spawning: The Egg Polarization Index or PI
Frank A. Chapman and Joel P. Van Eenennaam
FA-153 describes a simple field and laboratory test used to assist in predicting ovulatory response in female sturgeon, which uses the egg size and position of the nucleus in the cytoplasm as indicators — the principle behind it, materials needed, biopsy procedure, and how to determine the egg polarization index.