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Understanding Insecticide Modes of Action and Resistance Management in Florida Horticulture

Hugh Smith, Adam Dale, and Julien Beuzelin

This publication is designed to help UF/IFAS Extension agents and members of the public understand insecticide modes of action and resistance management as they pertain to vegetables, field crops, turf, and ornamental plants.

Mode of Action

The mode of action of an insecticide is the way the insecticide kills an insect. Other definitions include the “means by which a toxin affects the anatomy, physiology, or biochemistry of an organism (Pedigo 2002),” and “the action of an insecticide at its target site (PCT 2021).” Table 1 lists important mode of action groups for insecticides associated with Florida horticulture. It includes examples of trade names by which these insecticides are commonly known in vegetable, row crop, turfgrass, and ornamental plant production and management. Table 1 also indicates pest groups commonly targeted by different mode of action groups. The Insecticide Resistance Action Committee (IRAC) ( is an international association that defines modes of action and assigns them a number and letter to facilitate resistance management.For example, the neonicotinoids (4A), sulfoximines (4C), and butenolides (4D) are all in group 4, so they all have the same mode of action. Structural differences in the chemical compounds result in these insecticides interacting differently with the target site and being placed into subgroups (A, C, and D).

The mode of action of an insecticide is determined by its active ingredient, which is the chemical compound responsible for the toxic effect. Some mode of action groups such as the pyrethroids (3A) include dozens of active ingredients, while others such as the butenolides (4D) presently contain only one active ingredient (flupyradifurone). Often active ingredients in the same mode of action group are effective against a similar group of target pests. For example, the neonicotinoid (4A) insecticides acetamiprid, imidacloprid, and thiamethoxam are primarily used against piercing-sucking insects such as whiteflies, aphids, and mealybugs. However different active ingredients within the same mode of action group may have distinct targets. Abamectin is a group 6 insecticide that is primarily used against mites and leafminers, while emamectin benzoate, also in group 6, is primarily effective against caterpillars and beetles.

Insecticide Resistance

Repeated exposure to the same mode of action can result in the development of insect and mite populations that are resistant to that mode of action. IRAC defines resistance as “a heritable change in the sensitivity of a pest population that is reflected in the repeated failure to achieve the expected level of control when used according to the label recommendation for that pest species.” The risk of insecticide resistance increases when successive generations of a pest are exposed to the same mode of action. Some individuals within any given pest population may be resistant to an insecticide because of genetic (“heritable”) factors. Each successive application of that insecticide reduces the susceptible portion of the pest population, so the insecticide-resistant portion gradually predominates if the same mode of action is repeatedly applied. Therefore, it is essential that pesticide applicators be familiar with the mode of action of any insecticide or miticide they apply to offset the development of resistance.

Treatment Intervals

The treatment interval approach to managing insecticide resistance involves grouping modes of action according to the target pest’s generation time or life span. The treatment interval is based on an estimate of the pest’s life span. The treatment interval approach is used to avoid treating subsequent generations of a pest population with the same mode of action. The same mode of action can be applied more than once during a treatment interval, but during the following treatment interval, a different mode of action should be used, or no insecticides should be applied.

For example, some insecticide labels use a thirty-day treatment interval for diamondback moth (Plutella xylostella) management, because thirty days is a good estimate of the diamondback moth’s life span. Insect and mite species vary in the time required to complete their life cycle, and mites tend to have shorter generation times than most insects.

For the purposes of resistance management, modes of action should be grouped by number (main group), not letter (subgroup). For example, a neonicotinoid and a butenolide insecticide could be used within the same treatment interval, but no group 4 insecticide should be used in the following treatment interval.

There are many sources of information on pest life cycles, including the University of Florida’s Featured Creatures series ( The publication “Managing Resistance to Diamide Insecticides in Florida Tomato” ( provides a more detailed description of treatment intervals. Remember that soon after a pest has become established on a crop or plant, pest generations will overlap. It is impossible to completely avoid treating successive generations of a pest with the same modes of action. However, the treatment interval approach provides a framework for reducing the likelihood that resistance will develop. Generation times for a given pest are influenced by temperature and host plant, but for the sake of planning, fixed treatment intervals are used for specific pests.

Key insecticide classes for Florida row crops, vegetables, turfgrasses, and ornamental plants

The IRAC Mode of Action group number for each insecticide class is indicated in parentheses. Bolded phrases are explained in the glossary.

For a comprehensive list and description of all currently recognized insecticide modes of action, consult

  • Carbamate (1A) and organophosphate (1B) insecticides are broad spectrum insecticides, meaning they will kill most insect groups regardless of their mouthparts or metamorphosis. These insecticides interfere with pathways in the nervous system that are important for animals, including humans, as well as insects, and so can pose a risk to applicators and field workers. Carbamate and organophosphate insecticides mostly work by contact, although there are some translaminar and systemic insecticides in these two groups. Some carbamates, such as methomyl, and organophosphates, including naled, are restricted-use insecticides.
  • Pyrethroids (3A) are broad spectrum nerve poisons that work by contact. These are among the most widely used insecticides and are synthetic versions of pyrethrins, insecticidal compounds produced by the plant Chrysanthemum cinerariifolium. Synthetic insecticides tend to have a longer residual efficacy than naturally occurring compounds, which tend to break down quickly when exposed to sunlight and the elements. Pyrethrins are among the insecticides that can be used in certified organic crop production. Many pyrethroids are restricted-use insecticides.
  • Neonicotinoids (4A), sulfoximines (4C) and butenolides (4D) are all nicotinic acetylcholine agonists, and so share the same mode of action. Group 4 insecticides are all systemic, and primarily target piercing-sucking insects through ingestion exposure. There are concerns about the impacts of neonicotinoid insecticides on pollinator health.
  • Spinosyns (5) include spinetoram and spinosad and are primarily used to manage thrips, leafminers, and caterpillars. The active ingredient in Entrust is spinosad, and this formulation is labeled for use in certified organic production. Spinosyns are translaminar and have a mode of action similar to neonicotinoids.
  • Avermectins (6) are used against several distinct pest groups. Abamectin, an insecticide-miticide, is one of the most widely used materials to manage spider mites (Tetranychus urticae and other species) and Liriomyza leafminers.
  • Pyriproxyfen (7C) is a juvenile hormone mimic that interferes with the processes by which insects hatch from the egg and develop from one larval or nymphal stage to the next. Juvenile hormone mimics are insect growth regulators and do not affect adult insects other than to reduce the viability of eggs produced by exposed females. Pyriproxifen is used primarily against whiteflies, scale insects, and ants, with some activity against caterpillars. Pyriproxifen has translaminar activity.
  • Chordotonal modulators (9) interfere with the functioning of stretch receptors in insects that influence movement and feeding. They are translaminar and are primarily used against aphids and whiteflies.
  • Mite growth inhibitors (10) interfere with the ability of mites to form chitin, a key ingredient in the exoskeleton of arthropods (Nauen and Smagghe 2006). Mite growth regulators are effective against egg and protonymph spider mites. They function by contact.
  • Bacillus thuringiensis (11A) products consist of the B. thuringiensis (Bt) bacterium and crystal proteins, called delta endotoxins, produced by the naturally occurring bacterium. These proteins bind to receptor sites in the stomach when ingested by a target insect, causing the stomach wall to rupture. In agriculture, Bt products are used primarily against caterpillars. The two most used subspecies of Bt are aizawi and kurstaki. The Bt subspecies israelensis is used against fungus gnats (Bradysia spp.). Most formulations of Bt are registered for use in certified organic crop production, but some (for example Crymax) are not. Bt products are very specific to the pests they target because other organisms lack the binding sites in the stomach area that are affected by the crystal protein.
  • Benzoylureas (15) are insect growth regulators that interfere with the insect’s ability to form chitin. They are used against a limited number of insect groups, including caterpillars, beetle larvae, grasshoppers, and psyllids. Adult stages are not affected. Group 15 insecticides function differently from insect growth regulators in other mode of action groups. They function by contact.
  • Buprofezin (16) is an insect growth regulator that interferes with the insect’s ability to form chitin. It is used to manage leafhoppers, planthoppers, whiteflies, scale insects, and mealybugs. Only the immature (nymphal) stages of the pest are affected. Group 16 insecticides function differently from insect growth regulators in other mode of action groups. Buprofezin functions primarily by contact.
  • Cyromazine (17) disrupts molting, the progression from one life stage to the next, in dipteran (fly) leafminer (Liriomyza spp.) larvae. It is also labeled for management of Colorado potato beetle (Leptinotarsa decemlineata) larvae in some crops. It is a contact insecticide.
  • Diacylhydrazines (18) interfere with the functioning of ecdysone, an insect prohormone involved in molting and metamorphosis. These insecticides are used to manage caterpillars and function by contact.
  • METI insecticides (20, 21) interfere with electron transport in the mitochondria. Important insecticides in this group include bifenazate, which is used to manage spider mites, as well as fenpyroximate and tolfenpyrad, which are used against several distinct pest groups. They are contact insecticides.
  • Indoxacarb (22A) is a sodium channel blocker, interfering with sodium metabolism. It is primarily used against caterpillars and mole crickets. It is a contact insecticide.
  • Lipid biosynthesis inhibitors (23) include spiromesifen and spirotetramat, which are used against mites, whiteflies and other pests. These materials have systemic activity.
  • Cyflumetofen (25) interferes with electron transport in the mitochondria. It is registered for use in a limited number of crops for management of spider mites and other mite groups. It is translaminar.
  • Diamides (28) interfere with the functioning of ryanodine receptors, which are important in the regulation of calcium. Most diamides are systemic. They vary with regard to the spectrum of pests they affect. Some diamides are commonly used for management of caterpillars and beetles (e.g., chlorantraniliprole, tetraniliprole), and others are used for management of piercing-sucking insects (e.g., cyantraniliprole).
  • Flonicamid (29) is a chordotonal modulator but functions differently from the group 9 insecticides. It has some systemic activity.

Insecticides and IPM

Insecticides are only one component of an integrated pest management program. Crops and plants are ideally monitored weekly to determine if pests are present and increasing to damaging numbers. Crops vary in their tolerance to pests. Farm and nursery managers vary in their tendencies to tolerate pest damage. Pests that do not transmit viruses can generally be tolerated at higher levels than insect vectors of disease.

For most of Florida’s high-value specialty crops, insecticides play a leading role in reducing damage and losses due to insects and mites. Conditions for pests are favorable almost year-round in Florida, and for this reason sanitation and clean culture play an important role in reducing habitat for pests. Harvested fields and plants in a nursery or greenhouse that are no longer marketable should be promptly destroyed so that they do not serve as a source of pests or disease. Commercial varieties of some crops have host plant resistance to viruses and other pathogens transmitted by arthropods.

Many pests in Florida are attacked by naturally occurring predators and parasitoids, and these natural enemies can help reduce pest populations. Some commercially available biological control agents can be purchased and released to help manage pests in some crop systems. Insecticidal soaps and oils can also be incorporated into insecticide rotations to help manage some pests. These biopesticides reduce arthropod numbers in ways that pests generally do not develop resistance to, and therefore are not assigned a mode of action number as conventional insecticides are. Most biopesticides are acceptable for use in certified organic crop production and have limited negative impacts on natural enemies and pollinators compared to conventional insecticides when used according to the label.

Insecticide use planning starts with the label. The label is the law. Many labels have pollinator protection instructions that restrict how and when the insecticide can be applied. Decisions regarding insecticide use are determined by many factors, including the seasonal stage of the crop or plant, risks to pollinators, days to harvest, and which insecticides have already been applied. The ability to manage arthropod pests primarily with insecticides is also influenced by the duration of the crop or plant. The likelihood that insecticide resistance will develop on perennial crops or landscape plants that are treated for years is higher than the risk of resistance developing on an annual vegetable crop that is only in the ground for a few months. However, vegetable crops in Florida are usually grown in multiple staggered plantings so that pest populations on a given farm or adjacent group of farms are exposed to insecticides for multiple generations over several months of the production season. This can also result in resistant pest populations over time. Familiarity with local conditions, grower practices and the seasonality of pest populations is useful for planning insecticide programs and offsetting the development of insecticide resistance.


Biological control agents are predators, parasitoids, nematodes, and pathogens that attack arthropod pests and can be mass reared and sold by commercial production facilities. Biocontrol agents are released or applied to production areas, often repeatedly, for management of arthropod pests.

Biopesticides include insecticidal soaps, oils, and botanical insecticides such as azadirachtin and neem products. Biopesticides also include microbial pathogens such as Bacillus thuringiensis (Bt) and the insect fungal pathogens Beauveria bassiana and Paecilomyces fumosoroseus that have been formulated to be applied as insecticides. Most biopesticides kill in a way that does not select for resistance and so are not assigned an IRAC mode of action number. The notable exceptionsare the Bt products, which have the mode of action number 11A. Biopesticides tend to have a shorter residual efficacy than conventional insecticides, and most are labeled for use in certified organic production.

Certified organic production complies with United States Department of Agriculture regulations for organic production ( Organic farming avoids synthetic inputs and emphasizes naturally evolved soil and crop processes (

Contact insecticides kill an insect when the insect comes in direct contact with the insecticide or its active residues.

Host plant resistance involves breeding heritable characteristics into crop plants, enabling them to produce marketable yields when exposed to levels of pest or disease that would cause economic losses in susceptible varieties.

Insect growth regulators are insecticides that interfere with the hormonal and enzymatic processes that direct the development of arthropods from one life stage to the next.

Metamorphosis is the process of changing in form through life stages to become an adult. Some insects like grasshoppers and stinkbugs pass through a simple or incomplete metamorphosis in which the immature stages (nymphs) look similar to the adult but lack wings. Insects like moths and beetles have complete metamorphosis and pass through four completely distinct life stages: egg, larva, pupa, and adult. The type of metamorphosis an insect pest passes through has a direct bearing on methods to monitor, identify, and manage the pest.

Mouthparts vary among different arthropod groups. Beetles, caterpillars, and grasshoppers are among the insects with chewing mouthparts. Aphids, whiteflies, and mites have piercing-sucking mouthparts. Thrips have rasping-sucking mouthparts. The type of mouthpart an insect has influences the damage it causes and the likelihood that it will ingest a specific type of insecticide.

Natural enemies of an insect or mite are the naturally occurring predators, parasitoids, nematodes, and pathogens (diseases) that attack and kill it in nature. Pesticide selection and application practices can be modified to limit impacts on natural enemies.

Parasitoids are insects that lay eggs inside or on other host insects. The parasitoid egg hatches and the parasitoid larva completes its development inside the insect host, initially feeding on host fluids but eventually feeding on the host’s tissue and killing it. The parasitoid larva then emerges from the host insect to complete its development, often forming a pupa within or on the host carcass. Most parasitoids are wasps or flies. Wasps use their ovipositor to insert the egg inside the host. Flies lack an ovipositor, and so lay their eggs on the body of the host. Parasitic fly larvae must burrow into the host to complete their development after hatching from the egg.

Pollinator health refers to the survival, reproduction, and abundance of pollinators. Pollinator health has become a global concern in recent decades due to the decline in populations of western honeybees (Apis mellifera) and many native bee species. Insecticides are one of several factors associated with these population declines.

Predators are insects and mites that attack and feed upon other arthropods, consuming multiple prey during their life cycle.

Residual efficacy refers to the period in days or weeks after application that an insecticide continues to reduce populations of a given pest.

Restricted-use insecticides can only be used by a certified pesticide applicator or under the supervision of a certified applicator. Insecticides are grouped in this category because of risks associated with human and environmental health.

Systemic insecticides enter treated plants through the roots or foliage and are distributed within the plant via the vascular system. Systemic insecticides tend to have a longer residual efficacy than contact or translaminar insecticides.

Translaminar insecticides can move from the side of the plant leaf where they are applied to the other side of the leaf and are also referred to as “locally systemic.” Translaminar insecticides tend to have a longer residual efficacy than contact insecticides.


(IRAC) Insecticide Resistance Action Committee. 2021.

Nauen, R., and G. Smagghe. 2006. “Mode of Action of Etoxazole.” Pest Management Science 62:379–82.

(PCT) Pest Control Technology. 2021. Accessed Sept 30, 2021.

Pedigo, L. 2002. Entomology and Pest Management, fourth edition. Prentice Hall, Upper Saddle River, New Jersey, USA.

Table 1. Important insecticide classes and their mode of action with example active ingredients, trade names, and target pests.


Chemical Class†

Mode of action

Example active ingredients

Horticultural trade name examples

Turf/ornamental trade name examples

Target Pests



Acetylcholinesterase inhibitor


Sevin 4F


Sevin SL

Broad spectrum


Lannate SP




Acetylcholinesterase inhibitor



Orthene, Precise

Broad spectrum



Dursban 50W





Diazinon AG500



Malathion 5EC

Malathion 5EC


Dimethoate 4EC

Dimethoate 4E









Sodium channel modulator



Brigade 2 EC, many generics


Talstar S,

Bifen XTS, many more

Broad spectrum



Decathlon, Tempo


Baythroid XL

Tempo Ultra


Asana XL



Warrior II

Demon WP, Demand CS





Mustang Maxx

Sevin, Triple Crown T&O (plus bifenthrin, imidacloprid)









Astro, Permethrin



Sodium channel modulator



Pyganic, Tersus

Broad spectrum



Nicotinic acetylcholine receptor competitive modulator




Piercing sucking insects, some beetles, and some thrips



Arena, Aloft (plus bifenthrin)



Safari, Zylam, Transtect


Admire Pro, many generics

Merit, Marathon, others


Actara, Platinum

Meridian, Flagship



Same as neonicotinoids


Closer, Transform

XXpire (+spinetoram)

Piercing sucking insects



Same as neonicotinoids


Sivanto Prime, Altus


Piercing sucking insects and some thrips



Nicotinic acetylcholine receptor allosteric modulator – Site I


Radiant SC

XXpire (+sulfoxaflor)

Thrips, caterpillars, and leafminers


Entrust, Blackhawk

Conserve, Entrust



Glutamate-gated chloride channel allosteric modulator



Agri-Mek, generics


AvidLucidDivanemAward II,


Varies according to active ingredient: check label.

emamectin benzoate







Juvenile hormone mimic





Juvenile stages of various pests including whiteflies, caterpillars, and ants. Check label.


Pyridine azomethine derivatives

Chordotonal organ TRPV channel modulator







Primarily aphids and whiteflies






Chordotonal organ TRPV channel modulator


Sefina, Versys




Mite growth inhibitors affecting CHS1


Savey 50 DF

Hexygon DF

Spider mites



Mite growth inhibitors affecting CHS1



Beethoven TR



Bacillus thuringiensis and the insecticidal proteins they produce

Microbial disruptor of insect midgut membrane

Bacillus thuringiensis aizawi strain


XenTari, Agree WG







B. thuringiensis galleriae


grubGONE! G


B. thuringiensis israelensis




B. thuringiensis kurstaki strain

Dipel DF, Javelin WG

Dipel Pro DF




Inhibitors of chitin biosynthesis affecting CHS1







Caterpillars and juvenile stages of some beetles, thrips, whiteflies, and other sucking insects


Dimilin 2L

Dimilin 4L



Inhibitors of chitin biosynthesis, Type 1



Talus 70DF

Juvenile stages of whiteflies, scales, mealybugs, planthoppers, and leafhoppers



Moulting disruptor, dipteran




Fly larvae and Colorado potato beetle larvae



Ecdysone receptor agonist


Confirm 2F

Intrepid 2F



Intrepid 2F

Confirm 2F


Mitochondrial complex III electron transport inhibitors


Amdro ProExtinguish

Amdro ProExtinguish

Fire ants



Mitochondrial complex III electron transport inhibitors


Kanemite 15 SC


Spider mites and broad mites



Mitochondrial complex III electron transport inhibitors


Acramite 50WS




Spider mites


METI acaricides and insecticides

Mitochondrial complex I electron transport inhibitor




Broad mites, rust mites, spider mites, and whiteflies


Portal XLO




Hachi-Hachi SC



Voltage-dependent sodium channel blockers



Avaunt eVo



Caterpillars, fire ants, and mole crickets


Tetronic and tetramic acid derivatives

Inhibitors of acetyl COA carboxylase










Whiteflies, russet mites, spider mites, and others






Mitochondrial complex II electron transport inhibitors




Spider mites



Ryanodine receptor modulators


Coragen, Vantacor



Caterpillars, leafminers, some piercing sucking insects, and turf weevils


Verimark, Exirel










Chordotonal organ modulators – undefined target site



Beleaf, Carbine



† If the group is represented by only one active ingredient, or if primarily one active ingredient is pertinent for Florida crop production, the active ingredient is listed here as well as in the active ingredient column.


Peer Reviewed

Publication #DNY-2087

Release Date:November 29, 2022

Related Experts

Dale, Adam G.


University of Florida

Beuzelin, Julien


University of Florida

Smith, Hugh A.


University of Florida

Related Topics

Fact Sheet
General Public

About this Publication

This document is ENY-2087, one of a series of the Entomology and Nematology Department, UF/IFAS Extension. Original publication date November 2022. Visit the EDIS website at for the currently supported version of this publication.

About the Authors

Hugh Smith, associate professor, Entomology and Nematology Department, UF IFAS Gulf Coast Research and Education Center; Adam Dale, associate professor, Entomology and Nematology Department; and Julien Beuzelin, assistant professor; Entomology and Nematology Department, UF/IFAS Everglades Research and Education Center; UF/IFAS Extension, Gainesville, Florida 32611.


  • Hugh Smith